抗体微球交联原理

Proteins bind to sulfate (S) or carboxylatemodified (Carboxylate-modifi ed) particles (magnetic and nonmagnetic) by adsorption.Adsorption is mediated by hydrophobic and ionic interactions between the protein and the surface of the particles.Adsorption of proteins to particles occurs rapidly due to the microparticle surface free energy. Proteins may also be covalently attached to the surface of Carboxylate-modifi ed particles. Carboxyl groups on the particles, activated by the watersoluble carbodiimide 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC), react with free amino groups of the adsorbed protein to form amide bonds. For magnetic particles, we recommend following a covalent coupling procedure. Performing covalent coupling with the direct EDAC procedure is universally useful. If exposure of a protein to EDAC is discovered to be harmful to the protein, then a pre-activation (active ester) step prior to introducing the protein is an alternative for successful covalent coupling.